Coding
Part:BBa_K4162001
Designed by: Weiwen Chen Group: iGEM22_Fudan (2022-08-21)
StayGold = (n2)oxStayGold(c4)v2.0
Introduction
A new version of StayGold[1], which gives a higher expression level than the original version, and is over one order of magnitude more photostable than EGFP. The StayGold gene has been codon optimization for exogenous expression.
Worth to Notice
This biobrick is the version of StayGold available on Addgene.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 638
Illegal BsaI.rc site found at 658
- ↑ A highly photostable and bright green fluorescent protein. Hirano M, Ando R, Shimozono S, Sugiyama M, Takeda N, Kurokawa H, Deguchi R, Endo K, Haga K, Takai-Todaka R, Inaura S, Matsumura Y, Hama H, Okada Y, Fujiwara T, Morimoto T, Katayama K, Miyawaki A. Nat Biotechnol, 2022 Jul;40(7):1132-1142. PMID:35468954
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Categories
Parameters
None |